|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42150||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7904
- Total vector size (bp) 9793
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Tetracycline
Growth instructionspCC1Fos-based 'CopyControl' construct enabling transient induction of copy-number
Copy numberLow Copy
Single-copy vector under non-induced condition enabling stable propagation of RT-cassette. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNH034 was a gift from Colin Dolphin (Addgene plasmid # 42150 ; http://n2t.net/addgene:42150 ; RRID:Addgene_42150)
For your References section:A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT. BMC Biotechnol. 2013 Jan 3;13(1):1. 10.1186/1472-6750-13-1 PubMed 23281894