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pRc/CMV2 Dvl-2-Myc
(Plasmid #42194)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42194 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRc/CMV2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dvl 2
  • Alt name
    dishevelled 2, dsh homolog
  • Alt name
    Dvl2
  • Alt name
    Dvl-2
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_007888.3 NP_031914.3
  • Entrez Gene
    Dvl2
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dvl-2 sequence was amplified by PCR using a pBluescript IISK plasmid containing the entire coding sequence of mouse Dvl-2 (from Daniel J. Sussman, University of Maryland School of Medicine, Baltimore, MD) as the template.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The resulting PCR described above encompasses amino acids 1-679 of the current NCBI sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRc/CMV2 Dvl-2-Myc was a gift from Robert Lefkowitz & Shin-ichi Yanagawa (Addgene plasmid # 42194 ; http://n2t.net/addgene:42194 ; RRID:Addgene_42194)
  • For your References section:

    Characterization of mouse dishevelled (Dvl) proteins in Wnt/Wingless signaling pathway. Lee JS, Ishimoto A, Yanagawa S. J Biol Chem. 1999 Jul 23;274(30):21464-70. 10.1074/jbc.274.30.21464 PubMed 10409711