pBS-KSTbx2.8.2
(Plasmid #42626)

• Depositing Lab: Virginia Papaioannou
• Publication: Chapman et al Dev Dyn. 1996 Aug;206(4):379-90.

Backbone

• Vector backbone: pBluescript II-KS(+)
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tbx2
• Alt name: T-box 2
• Species: M. musculus (mouse)
• Insert Size (bp): 2000
• Entrez Gene: Tbx2
• Promoter: T3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Received from Lee Silver.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Bollag et al. (1994) Nature Genetics 7:383-389.