pFRT/FLAG/HA-DEST LIN28B
(Plasmid #43798)

• Depositing Lab: Thomas Tuschl
• Publication: Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261/rna.036491.112. Epub 2013 Mar 12.

Backbone

• Vector backbone: pFRT/FLAG/HA-DEST
• Backbone size w/o insert (bp): 5372
• Total vector size (bp): 6325
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LIN28B
• Species: H. sapiens (human)
• Insert Size (bp): 753
• GenBank ID: 389421
• Entrez Gene: LIN28B (a.k.a. CSDD2)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG-HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGHR

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the Flag and HA tags are not in frame in the depositor's assembled sequence, but Addgene's quality control sequence shows they are in frame.