|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||43798||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5372
- Total vector size (bp) 6325
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
Insert Size (bp)753
Entrez GeneLIN28B (a.k.a. CSDD2)
- Promoter CMV
/ Fusion Protein
- FLAG-HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note that the Flag and HA tags are not in frame in the depositor's assembled sequence, but Addgene's quality control sequence shows they are in frame.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFRT/FLAG/HA-DEST LIN28B was a gift from Thomas Tuschl (Addgene plasmid # 43798 ; http://n2t.net/addgene:43798 ; RRID:Addgene_43798)
For your References section:Identification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition. Hafner M, Max KE, Bandaru P, Morozov P, Gerstberger S, Brown M, Molina H, Tuschl T. RNA. 2013 May;19(5):613-26. doi: 10.1261/rna.036491.112. Epub 2013 Mar 12. 10.1261/rna.036491.112 PubMed 23481595