This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

sZF7
(Plasmid #43838)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 43838 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sZF7
  • Species
    Synthetic
  • Insert Size (bp)
    315
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA Flag (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer CMV_F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sZF7 was a gift from George Church (Addgene plasmid # 43838)
  • For your References section:

    Barcoding cells using cell-surface programmable DNA-binding domains. Mali P, Aach J, Lee JH, Levner D, Nip L, Church GM. Nat Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407. 10.1038/nmeth.2407 PubMed 23503053