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pCR3-FLAG-TRAF6-C70A
(Plasmid #43926)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 43926 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCR3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6690
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNF Receptor-Associated Factor 6
  • Alt name
    TRAF6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1650
  • Mutation
    C70A
  • GenBank ID
    NM_009424.2
  • Entrez Gene
    Traf6 (a.k.a. 2310003F17Rik, AI851288, C630032O20Rik)
  • Tag / Fusion Protein
    • 2x FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H. Nakano
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3-FLAG-TRAF6-C70A was a gift from Bryant Darnay (Addgene plasmid # 43926 ; http://n2t.net/addgene:43926 ; RRID:Addgene_43926)
  • For your References section:

    Site-specific Lys-63-linked tumor necrosis factor receptor-associated factor 6 auto-ubiquitination is a critical determinant of I kappa B kinase activation. Lamothe B, Besse A, Campos AD, Webster WK, Wu H, Darnay BG. J Biol Chem. 2007 Feb 9;282(6):4102-12. Epub 2006 Nov 29. 10.1074/jbc.M609503200 PubMed 17135271