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pNAHA
(Plasmid #44169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44169 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 44169-D50 50 μg of DNA in Tris buffer $495
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DNA 44169-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pVITRO2-mcs
  • Backbone manufacturer
    InvivoGen
  • Backbone size w/o insert (bp) 6321
  • Total vector size (bp) 9434
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hemagglutinin gene
  • Alt name
    influenza HA
  • Species
    Influenza A/FPV/Rostock/1934, subtype H7
  • Insert Size (bp)
    1742
  • GenBank ID
    M24457.1
  • Promoter CMV enhancer/human Ferritin light chain promoter

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SgrAI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    neuraminidase gene
  • Alt name
    influenza NA
  • Species
    influenza A/Puerto Rico/8/34, subtype N1
  • Insert Size (bp)
    1362
  • Mutation
    T55A
  • GenBank ID
    CY033579.1
  • Promoter SV40 enhancer/human ferritin heavy chain promoter

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The hemagglutinin gene was obtained from Dr Francois-Loic Cosset (Laboratoire de Vectorologie Retrovirale et Therapie Genique, Lyon, France)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that a T55A mutation was identified in the N1 sequence.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNAHA was a gift from John Olsen (Addgene plasmid # 44169 ; http://n2t.net/addgene:44169 ; RRID:Addgene_44169)

  • For your References section:

    High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors. Patel M, Giddings AM, Sechelski J, Olsen JC. J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695. 10.1002/jgm.2695 PubMed 23319179
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