Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44266||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4737
- Total vector size (bp) 9872
Modifications to backboneCMV promoter replaced with murine K15 promoter (-4.8) fragment
Vector typeMammalian Expression, Mouse Targeting ; Fluorescence microscopy
Selectable markersNeomycin (select with G418) ; EGFP
Growth in Bacteria
Gene/Insert nameK15 promoter (-4.8)
Alt nameKrt1-15 promoter
Alt namemouse keratin 15 promoter
Alt nameKrt15 keratin 15
SpeciesM. musculus (mouse)
Insert Size (bp)4961
MutationContains the murine K15 promoter (-4.8) fragment
GenBank IDNC_000077.6 AF542050
Entrez GeneKrt15 (a.k.a. RP23-217I3.2, AI528832, K15, Krt1-15)
- Promoter murine K15 promoter (-4.8) fragment
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBR322ori-F
- 3′ sequencing primer EGFP-N; SV40-pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Alternate plasmid name: pEGFP-N2 K15
The K15 promoter was cloned from C57 BL/SJ mouse genomic DNA (isolated from tail) by PCR using the Supermix High Fidelity Kit (GIBCO). The sequences of the forward and reverse primers were
respectively. XhoI and EcoRI restriction sequences were added to the 5' end of forward and reverse primers respectively for subsequent cloning of the PCR product into PBK/CMV vector (Stratagene). The promoter fragment was then subcloned into pGEGFP-N2
The entire K15 promoter-EGFP transgene can be released by digestion with XhoI/AflII.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:K15-EGFP was a gift from George Cotsarelis (Addgene plasmid # 44266 ; http://n2t.net/addgene:44266 ; RRID:Addgene_44266)
For your References section:Capturing and profiling adult hair follicle stem cells. Morris RJ, Liu Y, Marles L, Yang Z, Trempus C, Li S, Lin JS, Sawicki JA, Cotsarelis G. Nat Biotechnol. 2004 Apr;22(4):411-7. Epub 2004 Mar 14. 10.1038/nbt950 PubMed 15024388
Map uploaded by the depositor.