pBH6119-toxTpro-gfp
(Plasmid #44388)

• Depositing Lab: Victor DiRita
• Publication: Anthouard et al MBio. 2013 Aug 6;4(4). pii: e00403-13. doi: 10.1128/mBio.00403-13.

Backbone

• Vector backbone: pBH6119
• Vector type: Bacterial Expression
• Selectable markers: thy+

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: toxTpro
• Alt name: toxT promoter
• Alt name: (-172) to (+45) toxT promoter fragment
• Species: Vibrio cholerae
• Insert Size (bp): 216
• Promoter: toxT

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GTA TAG CAA AGC ATA TTC AG
• 3′ sequencing primer: AAA TAA ACG CAG AGA GC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The pBH6119 plasmid was obtained from Michele Swanson's lab, at the University of Michigan.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The toxT promoter drives expression of a stable form of GFPmut3. Please note that although this plasmid can be propagated in E. coli, it is a Vibrio cholerae vector and should be used in a Vibrio strain for experiments.