pBabe-Myc-TRFcH
(Plasmid #44574)

• Depositing Lab: Eros Lazzerini Denchi
• Publication: Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature11873.

Backbone

• Vector backbone: pBabe
• Total vector size (bp): 6432
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: chimeric TRF
• Species: H. sapiens (human)
• Insert Size (bp): 1242
• Mutation: TRF2 Hinge domain (aa245-443) replaced by that of TRF1 (aa265-376)
• GenBank ID: NP_005643.1 for TRF2 NP_059523.2 for TRF1
• Entrez Gene: TERF2 (a.k.a. TRBF2, TRF2)
• Entrez Gene: TERF1 (a.k.a. PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBabe 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TRF2 construct in which the Hinge domain (aa254-443 when compared to NP_005643.1) was replaced with Hinge domain of TRF1 (aa265-376 when compared to NP_059523.2)