KFS2MT
(Plasmid
#44624)
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Depositing Lab
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Depositing OrganizationSanford Burnham Prebys Medical Discovery Institute
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 44624-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 44624-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGEM-1
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Vector typeMouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namekeratin 18 gene
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SpeciesH. sapiens (human)
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Entrez GeneKRT18 (a.k.a. CK-18, CYK18, K18)
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Tag
/ Fusion Protein
- polyomavirus middle T antigen (PyMT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
K18 gene with trimer poly A signals flanked on both sides by two loxp seq., followed by IRES seq and PyMT gene. Cre removes termination sequences permitting K18 gene to be transcribed and spliced normally with PyMT gene being expressed from second translational reading frame.
Human K18 gene from pGC1853 (Addgene #44619).
DNA (Catalog # 44624-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 44624-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KFS2MT was a gift from Robert Oshima (Addgene plasmid # 44624 ; http://n2t.net/addgene:44624 ; RRID:Addgene_44624) -
For your References section:
Differential sensitivity of mouse epithelial tissues to the polyomavirus middle T oncogene. Cecena G, Wen F, Cardiff RD, Oshima RG. Am J Pathol. 2006 Jan;168(1):310-20. 10.2353/ajpath.2006.050443 PubMed 16400032
Map uploaded by the depositor.
