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pFA6a-link-yEPAGFP-CaUra3
(Plasmid #44649)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44649 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yEPAGFP
  • Alt name
    yeast codon optimized photoactivatible GFP
  • Insert Size (bp)
    717

Resource Information

  • Terms and Licenses

Depositor Comments

This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.

PAGFP was made my mutagenizing yEGFP to the sequence published in "A photoactivatable GFP for selective photolabeling of proteins and cells." Patterson GH, Lippincott-Schwartz J. Science. 2002 Sep 13;297(5588):1873-7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-link-yEPAGFP-CaUra3 was a gift from Kurt Thorn (Addgene plasmid # 44649)