|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44649||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Alt nameyeast codon optimized photoactivatible GFP
Insert Size (bp)717
Terms and Licenses
This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
PAGFP was made my mutagenizing yEGFP to the sequence published in "A photoactivatable GFP for selective photolabeling of proteins and cells." Patterson GH, Lippincott-Schwartz J. Science. 2002 Sep 13;297(5588):1873-7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-link-yEPAGFP-CaUra3 was a gift from Kurt Thorn (Addgene plasmid # 44649)