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Measles V-FLAG
(Plasmid #44909)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44909 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF-FLAG
  • Backbone size w/o insert (bp) 5358
  • Total vector size (bp) 6200

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MeV
  • Insert Size (bp)
    900
  • GenBank ID
    Q9IC37.1
  • Entrez Gene
    P/V/C (a.k.a. MeVgp2, P)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer ??
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Measles V-FLAG was a gift from Curt Horvath (Addgene plasmid # 44909 ; http://n2t.net/addgene:44909 ; RRID:Addgene_44909)
  • For your References section:

    STAT protein interference and suppression of cytokine signal transduction by measles virus V protein. Palosaari H, Parisien JP, Rodriguez JJ, Ulane CM, Horvath CM. J Virol. 2003 Jul;77(13):7635-44. 10.1128/JVI.77.13.7635-7644.2003 PubMed 12805463