Skip to main content
Addgene
Open Menu

Hsp70-mplum
(Plasmid #45016)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45016 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 45016-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 45016-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pDEST-R4-R3
  • Backbone manufacturer
    Life Technologies/Invitrogen
  • Total vector size (bp) 4511
  • Modifications to backbone
    The attB1 site resulting from MultiSite Gateway recombination was lost owing to further modification in order to insert the beta-globin intron between the Hspa1b promoter and the mPlum coding sequence.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mPlum
  • Species
    Discosoma sp.
  • Insert Size (bp)
    681
  • GenBank ID
    AY786537.1
  • Promoter mouse Hspa1b

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer M13 forward (-20)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original mPlum cDNA for constructing our plasmid was initially provided by Dr Roger Y.Tsien (UCSD), and use was subject to their MTA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Wang L, Jackson WC, Steinbach PA, Tsien RY. Evolution of new nonantibody
proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A. 2004 Nov
30;101(48):16745-9.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hsp70-mplum was a gift from Anna Planas (Addgene plasmid # 45016 ; http://n2t.net/addgene:45016 ; RRID:Addgene_45016)

  • For your References section:

    In vivo imaging of induction of heat-shock protein-70 gene expression with fluorescence reflectance imaging and intravital confocal microscopy following brain ischaemia in reporter mice. de la Rosa X, Santalucia T, Fortin PY, Purroy J, Calvo M, Salas-Perdomo A, Justicia C, Couillaud F, Planas AM. Eur J Nucl Med Mol Imaging. 2013 Feb;40(3):426-38. doi: 10.1007/s00259-012-2277-7. Epub 2012 Nov 8. 10.1007/s00259-012-2277-7 PubMed 23135322
Related items:
Commonly requested with: