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pBS-Histone H1
(Plasmid #45083)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 45083 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript II SK(+)
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3800
  • Vector type
    RNAi ; in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Histone H1
  • Alt name
  • Alt name
  • Alt name
  • Species
    Schmidtea mediterranea
  • Insert Size (bp)
  • Promoter T7 sense, T3 antisense

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer M13 –20
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-Histone H1 was a gift from Phillip Newmark (Addgene plasmid # 45083 ; ; RRID:Addgene_45083)
  • For your References section:

    PIWI homologs mediate histone H4 mRNA localization to planarian chromatoid bodies. Rouhana L, Weiss JA, King RS, Newmark PA. Development. 2014 Jul;141(13):2592-601. doi: 10.1242/dev.101618. Epub 2014 Jun 5. 10.1242/dev.101618 PubMed 24903754