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FRas2-M Acceptor
(Plasmid #45149)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 45149 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5640
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    mRFP - Raf binding domain-mRFP
  • Alt name
  • Alt name
    Raf Binding Domain
  • Insert Size (bp)
  • Mutation
    lysine 65 changed to glutamine, lysine 108 mutated to alanine in raf binding domain
  • Tag / Fusion Protein
    • mRFP

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRas2-M Acceptor was a gift from Ryohei Yasuda (Addgene plasmid # 45149 ; ; RRID:Addgene_45149)
  • For your References section:

    An improved Ras sensor for highly sensitive and quantitative FRET-FLIM imaging. Oliveira AF, Yasuda R. PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 2013 Jan 14. 10.1371/journal.pone.0052874 PubMed 23349692