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(Plasmid #45152)


Item Catalog # Description Quantity Price (USD)
Plasmid 45152 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Keasling Lab
  • Backbone size w/o insert (bp) 3489
  • Total vector size (bp) 5883
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Mesoplasma florum
  • Insert Size (bp)
  • Promoter PLlacO-1
  • Tag / Fusion Protein
    • ssRA degradation (LVA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CCACGCCGAAACAAG
  • 3′ sequencing primer CGAAGGTGAGCCAGTGT
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Was obtained from Addgene
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLlacO-1-mfLon was a gift from Michel Maharbiz (Addgene plasmid # 45152 ; ; RRID:Addgene_45152)
  • For your References section:

    A genetic bistable switch utilizing nonlinear protein degradation. Huang DC, Holtz WJ, Maharbiz MM. J Biol Eng. 2012 Jul 9;6(1):9. 10.1186/1754-1611-6-9 PubMed 22776405