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(Plasmid #40127)


Item Catalog # Description Quantity Price (USD)
Plasmid 40127 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2097
  • Total vector size (bp) 2965
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    CI inducible sfGFP
  • Species
    E. Coli
  • GenBank ID
  • Promoter pRM
  • Tag / Fusion Protein
    • LVA ssrA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, BglII (not destroyed)
  • 3′ cloning site BamHI, XhoI (not destroyed)
  • 5′ sequencing primer GFP-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRM-GFP was a gift from Michel Maharbiz (Addgene plasmid # 40127 ; ; RRID:Addgene_40127)
  • For your References section:

    A genetic bistable switch utilizing nonlinear protein degradation. Huang DC, Holtz WJ, Maharbiz MM. J Biol Eng. 2012 Jul 9;6(1):9. 10.1186/1754-1611-6-9 PubMed 22776405