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pBabe_puro_DEST_Flag_TNIK
(Plasmid #45276)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45276 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-puro-flag-DEST
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNIK
  • Alt name
    TNIK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystems MHS1010-9203902
  • Terms and Licenses

Depositor Comments

The TNIK open reading frame was PCR amplified with BP flanked primers from the vector acquired from Open Biosystems and ligated into a pDONR223 vector in a BP Gateway ligation reaction. The TNIK open reading frame was subsequently ligated into the pBabe_puro_DEST_flag backbone in a Gateway LR ligation reaction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe_puro_DEST_Flag_TNIK was a gift from William Hahn (Addgene plasmid # 45276 ; http://n2t.net/addgene:45276 ; RRID:Addgene_45276)
  • For your References section:

    Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer drivers. Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P, Mesirov JP, Beroukhim R, Hahn WC. Cancer Discov. 2013 Jun 13. [Epub ahead of print] 10.1158/2159-8290.CD-12-0592 PubMed 23764425