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(Plasmid #45283)


Item Catalog # Description Quantity Price (USD)
Plasmid 45283 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    New England Biolabs, INC
  • Backbone size w/o insert (bp) 7477
  • Total vector size (bp) 7718
  • Vector type
    Bacterial Expression ; Protein Expression Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    contains aa20-113 of lacritin
  • GenBank ID
  • Entrez Gene
  • Promoter T7
  • Tag / Fusion Protein
    • Intein (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The last 25 amino acids of lacritin were deleted in this construct (aa 114-138).

Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence. The depositing laboratory indicates that these differences are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C25-pLAC was a gift from Gordon Laurie (Addgene plasmid # 45283 ; ; RRID:Addgene_45283)
  • For your References section:

    Restricted epithelial proliferation by lacritin via PKCalpha-dependent NFAT and mTOR pathways. Wang J, Wang N, Xie J, Walton SC, McKown RL, Raab RW, Ma P, Beck SL, Coffman GL, Hussaini IM, Laurie GW. J Cell Biol. 2006 Aug 28;174(5):689-700. Epub 2006 Aug 21. 10.1083/jcb.200605140 PubMed 16923831