Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pBbA5k-EPL123
(Plasmid #45361)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45361 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBbA5k
  • Backbone size w/o insert (bp) 6826
  • Total vector size (bp) 8021
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH1 w/ AcrAB knocked out
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AcrAB
  • Species
    Ecoli
  • Promoter LacUV5
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CAA AAG ATC TTT TAA GAA GGA GAT ATA CAT ATG AAC AAA AAC AGA GGG TTT ACG
  • 3′ sequencing primer CAG TGG TGA TGG TGA TGA TGA TGA TGA TCG ACA GTA TGG CTG TGC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5k-EPL123 was a gift from Aindrila Mukhopadhyay (Addgene plasmid # 45361 ; http://n2t.net/addgene:45361 ; RRID:Addgene_45361)
  • For your References section:

    Engineering microbial biofuel tolerance and export using efflux pumps. Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi MZ, Mukhopadhyay A. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. 10.1038/msb.2011.21 PubMed 21556065