pBEST-p15A-PLlacO1-deGFP-T500
(Plasmid
#45394)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBEST-Luc
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4486
- Total vector size (bp) 4486
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedeGFP
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Alt namedeGFP
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Speciese. coli
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Insert Size (bp)673
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GenBank ID
- Promoter PL-lacO1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site sphI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEST-p15A-PLlacO1-deGFP-T500 was a gift from Vincent Noireaux (Addgene plasmid # 45394 ; http://n2t.net/addgene:45394 ; RRID:Addgene_45394) -
For your References section:
Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. Shin J, Noireaux V. J Biol Eng. 2010 Jun 24;4:8. 10.1186/1754-1611-4-8 PubMed 20576148
