pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
(Plasmid #40019)

• Depositing Lab: Vincent Noireaux
• Publication: Shin et al J Biol Eng. 2010 Jun 24;4:8.

Backbone

• Vector backbone: pBEST-Luc
• Backbone manufacturer: Promega
• Total vector size (bp): 4486
• Modifications to backbone: pTacI promoter was removed and replaced by the bacteriophage Lambda promoter OR2-OR1-Pr, with one mutation made in this promoter. Untranslated region was removed and relplaced by UTR1, a powerful UTR (see reference related to this plasmid) Luc gene (firefly Luciferase) was removed and replaced by deGFP. A transcriptional terminator was added, called T500 (see reference related to this plasmid).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): KL740
• Growth instructions: Grow at 29C in strain KL740, LB medium.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: deGFP
• Alt name: eGFP-Del6-229
• Species: Synthetic
• Insert Size (bp): 678
• Promoter: OR2-OR1-Pr (bacteriophage Lambda with one mutation)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TATACCATGGAGCTTTTCACTGGCG
• 3′ sequencing primer: CGTGACCGCCGCCGGGATCTAACTCGAGCAAAG

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry