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(Plasmid #45788)


Item Catalog # Description Quantity Price (USD)
Plasmid 45788 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Modifications to backbone
    pTacI promoter was removed and replaced by pBAD-tetO1 (repressed by TetR). Untranslated region was removed and replaced by UTR1, a powerful UTR. Luc gene (firefly Luciferase) was removed and replaced by deGFP, ssrA tagged. A transcriptional terminator was added, called T500.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Species
  • Insert Size (bp)
  • Promoter pBAD-tetO1
  • Tag / Fusion Protein
    • ssrA degradation tag

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CATGGTGAAGACTATCGCAC
  • 3′ sequencing primer GAAGGAGCTGACTGGGTTGA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500 was a gift from Richard Murray (Addgene plasmid # 45788 ; ; RRID:Addgene_45788)