|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45777||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Modifications to backbonepTacI promoter was removed and replaced by PLlacO-1. Untranslated region was removed and replaced by UTR1, a powerful UTR. Luc gene (firefly Luciferase) was removed and replaced by deCFP. A transcriptional terminator was added, called T500.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)678
- Promoter PLlacO-1
- Cloning method Unknown
- 5′ sequencing primer CATGGTGAAGACTATCGCAC
- 3′ sequencing primer GAAGGAGCTGACTGGGTTGA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBEST-PLlacO-1-UTR1-deCFP-T500 was a gift from Richard Murray (Addgene plasmid # 45777 ; http://n2t.net/addgene:45777 ; RRID:Addgene_45777)