|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45779||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backbonepTacI promoter was removed and replaced by the bacteriophage Lambda promoter OR2-OR1-Pr, with one mutation made in this promoter. Untranslated region was removed and replaced by UTR1, a powerful UTR. Luc gene (firefly Luciferase) was removed and replaced by sigma factor 28. A transcriptional terminator was added, called T500.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Growth instructionsGrow at 29C in strain KL740, LB medium.
Copy numberLow Copy
Gene/Insert namesigma factor 28
Insert Size (bp)720
- Promoter OR2-OR1-Pr (bacteriophage Lambda with one mutation)
- Cloning method Unknown
- 5′ sequencing primer CATGGTGAAGACTATCGCAC
- 3′ sequencing primer TCTCATGTTTGACAGCTTATC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byVincent Noireaux, University of Minnesota
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500 was a gift from Richard Murray & Vincent Noireaux (Addgene plasmid # 45779 ; http://n2t.net/addgene:45779 ; RRID:Addgene_45779)