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pBEST-OR2-OR1-Pr-UTR1-deGFP-T500 Citations (17)

Originally described in: Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70.
Shin J, Noireaux V J Biol Eng. 2010 Jun 24;4:8.
PubMed Journal

Articles Citing pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

Articles
Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology. Sun ZZ, Hayes CA, Shin J, Caschera F, Murray RM, Noireaux V. J Vis Exp. 2013 Sep 16;(79):e50762. doi: 10.3791/50762. PubMed
Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system. Chemla Y, Ozer E, Schlesinger O, Noireaux V, Alfonta L. Biotechnol Bioeng. 2015 Aug;112(8):1663-72. doi: 10.1002/bit.25587. Epub 2015 Jun 16. PubMed
Mitochondrial 16S rRNA Is Methylated by tRNA Methyltransferase TRMT61B in All Vertebrates. Bar-Yaacov D, Frumkin I, Yashiro Y, Chujo T, Ishigami Y, Chemla Y, Blumberg A, Schlesinger O, Bieri P, Greber B, Ban N, Zarivach R, Alfonta L, Pilpel Y, Suzuki T, Mishmar D. PLoS Biol. 2016 Sep 15;14(9):e1002557. doi: 10.1371/journal.pbio.1002557. eCollection 2016 Sep. PubMed
Expression regulation by a methyl-CpG binding domain in an E. coli based, cell-free TX-TL system. Schenkelberger M, Shanak S, Finkler M, Worst EG, Noireaux V, Helms V, Ott A. Phys Biol. 2017 Mar 1;14(2):026002. doi: 10.1088/1478-3975/aa5d37. PubMed
Cell-free protein synthesis from non-growing, stressed Escherichia coli. Failmezger J, Rauter M, Nitschel R, Kraml M, Siemann-Herzberg M. Sci Rep. 2017 Nov 28;7(1):16524. doi: 10.1038/s41598-017-16767-7. PubMed
Electron transfer rate analysis of a site-specifically wired copper oxidase. Schlesinger O, Pasi M, Dandela R, Meijler MM, Alfonta L. Phys Chem Chem Phys. 2018 Feb 28;20(9):6159-6166. doi: 10.1039/c8cp00041g. PubMed
Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system. Shi X, Wu T, M Cole C, K Devaraj N, Joseph S. Sci Rep. 2018 Feb 22;8(1):3488. doi: 10.1038/s41598-018-21739-6. PubMed
A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs. Maxwell CS, Jacobsen T, Marshall R, Noireaux V, Beisel CL. Methods. 2018 Jul 1;143:48-57. doi: 10.1016/j.ymeth.2018.02.016. Epub 2018 Feb 24. PubMed

Associated Plasmids

Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens. Failmezger J, Scholz S, Blombach B, Siemann-Herzberg M. Front Microbiol. 2018 Jun 1;9:1146. doi: 10.3389/fmicb.2018.01146. eCollection 2018. PubMed
Bead-based assay for spatiotemporal gene expression control in cell-free transcription-translation systems. Finkler M, Ott A. Biotechniques. 2019 Jan;66(1):29-33. doi: 10.2144/btn-2018-0097. PubMed
Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors. Voyvodic PL, Pandi A, Koch M, Conejero I, Valjent E, Courtet P, Renard E, Faulon JL, Bonnet J. Nat Commun. 2019 Apr 12;10(1):1697. doi: 10.1038/s41467-019-09722-9. PubMed

Associated Plasmids

Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits. Contreras-Llano LE, Meyer C, Liu Y, Sarker M, Lim S, Longo ML, Tan C. Nat Commun. 2020 Jun 19;11(1):3138. doi: 10.1038/s41467-020-16900-7. PubMed
Full incorporation of the noncanonical amino acid hydroxylysine as a surrogate for lysine in green fluorescent protein. Finkler M, Ravanbodshirazi S, Grimm F, Hartz P, Ott A. Bioorg Med Chem. 2021 Jul 1;41:116207. doi: 10.1016/j.bmc.2021.116207. Epub 2021 May 9. PubMed
High-Efficiency Protection of Linear DNA in Cell-Free Extracts from Escherichia coli and Vibrio natriegens. Norouzi M, Panfilov S, Pardee K. ACS Synth Biol. 2021 Jun 23. doi: 10.1021/acssynbio.1c00110. PubMed

Associated Plasmids

A Methylation-Directed, Synthetic Pap Switch Based on Self-Complementary Regulatory DNA Reconstituted in an All E. coli Cell-Free Expression System. Worst EG, Finkler M, Schenkelberger M, Kurt O, Helms V, Noireaux V, Ott A. ACS Synth Biol. 2021 Oct 15;10(10):2725-2739. doi: 10.1021/acssynbio.1c00326. Epub 2021 Sep 22. PubMed
Cell-Free Biosensors and AI Integration. Soudier P, Faure L, Kushwaha M, Faulon JL. Methods Mol Biol. 2022;2433:303-323. doi: 10.1007/978-1-0716-1998-8_19. PubMed
Effects of DNA template preparation on variability in cell-free protein production. Romantseva E, Alperovich N, Ross D, Lund SP, Strychalski EA. Synth Biol (Oxf). 2022 Aug 13;7(1):ysac015. doi: 10.1093/synbio/ysac015. eCollection 2022. PubMed

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