A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs.
Maxwell CS, Jacobsen T, Marshall R, Noireaux V, Beisel CL
Methods. 2018 Jul 1;143:48-57. doi: 10.1016/j.ymeth.2018.02.016. Epub 2018 Feb 24. PubMed Article
Maxwell CS, Jacobsen T, Marshall R, Noireaux V, Beisel CL
Methods. 2018 Jul 1;143:48-57. doi: 10.1016/j.ymeth.2018.02.016. Epub 2018 Feb 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105683 | p70a-T7RNAP | Expresses T7 polymerase in cell-free TXTL. |
| 105684 | CSM160 | Plasmid containing an RFP dropout used to construct randomized PAM library |