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Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors.
Voyvodic PL, Pandi A, Koch M, Conejero I, Valjent E, Courtet P, Renard E, Faulon JL, Bonnet J
Nat Commun. 2019 Apr 12;10(1):1697. doi: 10.1038/s41467-019-09722-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
114597pBEAST-BenR"Strong constitutive expression of transcription factor, BenR, for cell-free expression"
114598pBEAST-pBen-sfGFP"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"
114599pBEST-HipOStrong constitutive expression of metabolic enzyme, HipO, for cell-free expression
114600pBEAST-J23101-CocE"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"
116917pBEAST-J23101-sfGFPStrong constitutive expression of super-folder GFP for cell-free expression
122694pBEAST-pBen-LucOutput expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression
126575pBEAST2-sfGFPStrong constitutive expression of super-folder GFP for cell-free expression

Antibodies from Article