pBEAST-pBen-Luc
(Plasmid #122694)

• Purpose: Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression
• Depositing Lab: Jerome Bonnet
• Publication: Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi: 10.1038/s41467-019-09722-9.

Backbone

• Vector backbone: pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
• Modifications to backbone: Two 40bp spacers were added to the each end of the insert to facilitate Gibson assembly cloning into more complex constructs. Upstream and downstream terminators (B0014 and B0015, respectively) we also added, each flanked by two 40bp spacers. The OR2-OR1-Pr promoter and UTR were replaced by the BenR-activated PBen and its native RBS, and deGFP was replace by firefly luciferase.
• Vector type: Synthetic Biology ; Cell-Free Protein Synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly luciferase
• Alt name: Luc
• Insert Size (bp): 1653

Resource Information

• Supplemental Documents:
  • pBEAST-pBen-Luc.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry