Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45399||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesB. taurus (bovine)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1-Rho-EGFP-ETSQVAPA was a gift from David Williams (Addgene plasmid # 45399 ; http://n2t.net/addgene:45399 ; RRID:Addgene_45399)
For your References section:Live-cell imaging evidence for the ciliary transport of rod photoreceptor opsin by heterotrimeric kinesin-2. Trivedi D, Colin E, Louie CM, Williams DS. J Neurosci. 2012 Aug 1;32(31):10587-93. doi: 10.1523/JNEUROSCI.0015-12.2012. 10.1523/JNEUROSCI.0015-12.2012 PubMed 22855808