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pEF-I-GFP GX
(Plasmid #45443)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45443 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF-GFP (Addgene Plasmid #11154)
  • Backbone manufacturer
    Connie Cepko lab (Harvard Medical School, Boston, MA)
  • Backbone size (bp) 7349
  • Modifications to backbone
    Converted to Gateway-compatible destination vector (gateway casette added). IRES inserted upstream of EGFP.
  • Vector type
    Mammalian Expression
  • Promoter human EF1α
  • Selectable markers
    EGFP
  • Tag / Fusion Protein
    • IRES EGFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pEF-GFP backbone from Connie Cepko (Harvard Medical Schoo, Boston, MA). IRES-GFP fragment from plasmid pBMN-I-GFP from Gary Nolan (Stanford, CA). RfC.1 cassette from Invitrogen (Carlsbad, CA).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: pEF1α-RfC.1-IRES-GFP

To construct this vector, the depositing lab used EcoRI and BsrGI to replace the EGFP in pEF–GFP (Addgene plasmid 11154) with the IRES-GFP fragment from plasmid pBMN-I-GFP (Addgene plasmid 1736), creating intermediate vector pEF1α-IRES-GFP.

Plasmid pEF1α-IRES-GFP was then converted to a Gateway-enabled destination vector with the Gateway Vector Conversion System (Invitrogen) by digesting with EcoRI, blunting the ends, and inserting Reading Frame Cassette C (RfC.1) gateway cassette.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-I-GFP GX was a gift from John Brigande (Addgene plasmid # 45443 ; http://n2t.net/addgene:45443 ; RRID:Addgene_45443)
  • For your References section:

    Functional auditory hair cells produced in the mammalian cochlea by in utero gene transfer. Gubbels SP, Woessner DW, Mitchell JC, Ricci AJ, Brigande JV. Nature. 2008 Sep 25. 455(7212):537-41. 10.1038/nature07265 PubMed 18754012