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Addgene

pBAD/HisB-iRFP682
(Plasmid #45454)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45454 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBAD/HisB
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4067
  • Total vector size (bp) 5018
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    iRFP682
  • Species
    Rhodopseudomonas palustris
  • Insert Size (bp)
    951
  • Mutation
    T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193K, F198Y, D202A, I203V, V254C, Y258F, A283V, K288T, N290Y relative to RpBphP2
  • GenBank ID
    KC991143
  • Promoter araBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBAD-fwd
  • 3′ sequencing primer pBAD-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/HisB-iRFP682 was a gift from Vladislav Verkhusha (Addgene plasmid # 45454 ; http://n2t.net/addgene:45454 ; RRID:Addgene_45454)
  • For your References section:

    Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755