pMMBrelAGent
(Plasmid #45474)

• Depositing Lab: Michele Swanson
• Publication: Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

Backbone

• Vector backbone: pMMB67EH
• Backbone manufacturer: Bagdasarian Lab
• Modifications to backbone: The Gentamycin cassette was Klenow treated and ligated into the DraI site in pMMB67EH. This inactivates the original amp cassette.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: relA
• Alt name: MB357
• Mutation: truncated at EcoRI/PstI sites of RelA, but metabolically active
• Promoter: ptac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: PstI (unknown if destroyed)
• 5′ sequencing primer: M13-Rev
• 3′ sequencing primer: pBAD-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a vector control for IPTG-indicible E. coli relA gene.