pCRII-Topo Cdh10 in situ probe
(Plasmid
#45638)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCRII-Topo
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4400
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Vector typein situ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdh10 in situ probe
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Alt nameCdh10
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Alt namecadherin 10, type 2 (T2-cadherin)
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SpeciesM. musculus (mouse)
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Insert Size (bp)434
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Mutationfragment contains nt 2628-3061 of Cdh10 (GenBank ID AF183946)
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Entrez GeneCdh10 (a.k.a. A830016G23Rik, C030003B10Rik, C030011H18Rik)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Rev, Sp6
- 3′ sequencing primer T7, M13 Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the SpeI restriction digest and T7 promoter for antisense probe generation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII-Topo Cdh10 in situ probe was a gift from Jeffrey Macklis (Addgene plasmid # 45638 ; http://n2t.net/addgene:45638 ; RRID:Addgene_45638)
Map uploaded by the depositor.
