|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45870||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4600
Vector typein situ
Growth in Bacteria
Gene/Insert nameCouptf1 in situ probe
Alt namenuclear receptor subfamily 2, group F, member 1
SpeciesM. musculus (mouse)
Insert Size (bp)619
Mutationcontains bp# 1755-2381 of NM_010151.2
Entrez GeneNr2f1 (a.k.a. COUP-TF1, COUP-TFI, COUPTFA, EAR-3, EAR3, Erbal3, SVP44, Tcfcoup1)
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Rev, Sp6
- 3′ sequencing primer T7, M13 Forward (Common Sequencing Primers)
Terms and Licenses
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 1755-2381 of NM_010151.2, not bp# 1635-2273 as indicated on plasmid map. The plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRII-Topo Couptf1 was a gift from Jeffrey Macklis (Addgene plasmid # 45870 ; http://n2t.net/addgene:45870 ; RRID:Addgene_45870)
Map uploaded by the depositor.