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pCRII-Topo Couptf1
(Plasmid #45870)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 45870 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4600
  • Vector type
    in situ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Couptf1 in situ probe
  • Alt name
  • Alt name
  • Alt name
    nuclear receptor subfamily 2, group F, member 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    contains bp# 1755-2381 of NM_010151.2
  • GenBank ID
  • Entrez Gene
    Nr2f1 (a.k.a. COUP-, COUP-T, COUP-TF1, COUP-TFI, COUPTFA, EAR-3, EAR3, Erb, Erbal3, SVP44, Tcfcou, Tcfcoup1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.

Please note that Addgene's sequencing results match bp# 1755-2381 of NM_010151.2, not bp# 1635-2273 as indicated on plasmid map. The plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-Topo Couptf1 was a gift from Jeffrey Macklis (Addgene plasmid # 45870 ; ; RRID:Addgene_45870)