pCRII-Topo Plexin D1 in situ probe
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45621||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4600
Vector typein situ
Growth in Bacteria
Gene/Insert namePlexin D1 in situ probe
Alt namePlexin D1
SpeciesM. musculus (mouse)
Mutationfragment contains bp# 5751-6406 of NM_026376.3
Entrez GenePlxnd1 (a.k.a. 6230425C21Rik, b2b1863Clo, b2b553Clo)
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Rev, Sp6
- 3′ sequencing primer T7, M13 Forward (Common Sequencing Primers)
Terms and Licenses
The Plexin D1 fragment was amplified by RT-PCR using the following primer pair:
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRII-Topo Plexin D1 in situ probe was a gift from Jeffrey Macklis (Addgene plasmid # 45621 ; http://n2t.net/addgene:45621 ; RRID:Addgene_45621)
For your References section:Fezl is required for the birth and specification of corticospinal motor neurons. Molyneaux BJ, Arlotta P, Hirata T, Hibi M, Macklis JD. Neuron. 2005 Sep 15;47(6):817-31. 10.1016/j.neuron.2005.08.030 PubMed 16157277
Map uploaded by the depositor.