mGbx2
(Plasmid #22092)

• Depositing Lab: Gail Martin
• Publication: Gail Martin plasmids (unpublished)

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GBX2
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Entrez Gene: Gbx2 (a.k.a. D130058E05Rik, Gbx-2, MMoxA, Stra7)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert contains the full coding sequence and has been used to make RNA probes.

For antisense probe linearize with HindIII and use T7 Polymerase. For sense probe linearize with ApaI or KpnI and transcribe with T3 RNA polymerase.