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pCAG-PLE-IRES-mCherry
(Plasmid #45766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45766 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 45766-D50 50 μg of DNA in Tris buffer $495
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DNA 45766-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 7231
  • Total vector size (bp) 8944
  • Modifications to backbone
    Added IRES, mCherry and WPRE to pCAGGS vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    esterase
  • Alt name
    PLE
  • Species
    Pig
  • Insert Size (bp)
    1701
  • Promoter CAG

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GAGAACGTGGTGGTGGTGG
  • 3′ sequencing primer CAGGGTCAGCCTCTCCTTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid designed for coexpression of PLE
and mCherry. PLE was subcloned upstream of mCherry and separated by an internal ribosome entry site (IRES) sequence in the pCAGGS vector.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-PLE-IRES-mCherry was a gift from Luke Lavis (Addgene plasmid # 45766 ; http://n2t.net/addgene:45766 ; RRID:Addgene_45766)

  • For your References section:

    Selective esterase-ester pair for targeting small molecules with cellular specificity. Tian L, Yang Y, Wysocki LM, Arnold AC, Hu A, Ravichandran B, Sternson SM, Looger LL, Lavis LD. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4756-61. doi: 10.1073/pnas.1111943109. Epub 2012 Mar 12. 10.1073/pnas.1111943109 PubMed 22411832
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