pAAV-myrSNAP
(Plasmid #60098)

• Purpose: Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional
• Depositing Lab: Gregory Jefferis
• Publication: Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. pii: 201411087.

Backbone

• Vector backbone: pAAV-CMV
• Backbone manufacturer: custom
• Backbone size w/o insert (bp): 4600
• Total vector size (bp): 6600
• Vector type: Mammalian Expression, Mouse Targeting, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: SNAP-tag
• Species: Synthetic
• Insert Size (bp): 546

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: SNAPfw
• 3′ sequencing primer: SNAPrev

Gene/Insert 2

• Gene/Insert name: histone-GFP
• Species: Synthetic
• Insert Size (bp): 1220

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: EGFPfw
• 3′ sequencing primer: EGFPrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry