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pCRII-Topo Semcap3
(Plasmid #45874)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45874 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCRII-Topo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4600
  • Vector type
    in situ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Semcap3 in situ probe
  • Alt name
    Semcap3
  • Alt name
    Pdzrn3
  • Alt name
    PDZ domain containing RING finger 3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    620
  • Mutation
    contains bp# 3184-3805 of AF127085.1
  • GenBank ID
  • Entrez Gene
    Pdzrn3 (a.k.a. 1110020C07Rik, AI429718, AL023082, LNX3, SEMACAP3, Semcap3)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.

Please note that Addgene's sequencing results match bp# 3184-3805 of AF127085.1, not bp# 3185-3804 as indicated on plasmid map. The plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-Topo Semcap3 was a gift from Jeffrey Macklis (Addgene plasmid # 45874 ; http://n2t.net/addgene:45874 ; RRID:Addgene_45874)