|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45874||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4600
Vector typein situ
Growth in Bacteria
Gene/Insert nameSemcap3 in situ probe
Alt namePDZ domain containing RING finger 3
SpeciesM. musculus (mouse)
Insert Size (bp)620
Mutationcontains bp# 3184-3805 of AF127085.1
Entrez GenePdzrn3 (a.k.a. 1110020C07Rik, AI429718, AL023082, LNX3, SEMACAP3, Semcap3)
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Rev, Sp6
- 3′ sequencing primer T7, M13 Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 3184-3805 of AF127085.1, not bp# 3185-3804 as indicated on plasmid map. The plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRII-Topo Semcap3 was a gift from Jeffrey Macklis (Addgene plasmid # 45874 ; http://n2t.net/addgene:45874 ; RRID:Addgene_45874)
Map uploaded by the depositor.