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pMRXIP GFP-Stx7
(Plasmid #45921)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45921 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMRXIP GFP-Ci2
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 7383
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    syntaxin 7
  • Alt name
    Stx7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    783
  • GenBank ID
    NM_003569.2
  • Entrez Gene
    STX7
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRXIP GFP-Stx7 was a gift from Noboru Mizushima (Addgene plasmid # 45921 ; http://n2t.net/addgene:45921 ; RRID:Addgene_45921)
  • For your References section:

    The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Itakura E, Kishi-Itakura C, Mizushima N. Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001. 10.1016/j.cell.2012.11.001 PubMed 23217709