pNMHCII-C2-GFP
(Plasmid #46043)

• Depositing Lab: Robert Adelstein
• Publication: Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-71. doi: 10.1074/jbc.M806574200. Epub 2009 Feb 23.

Backbone

• Vector backbone: pEGFP-N3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 10800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nonmuscle myosin heavy chain II-C, isoform C2
• Alt name: NMHCII-C, isoform C2
• Alt name: Myh14, isoform C2
• Species: M. musculus (mouse)
• Insert Size (bp): 6129
• GenBank ID: EF602040 NM_001271538
• Entrez Gene: Myh14 (a.k.a. 2400004E04Rik, II-C, NHMCII, NMHC II-C)
• Promoter: CMV immediate early
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-for: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: EGFP-N: CGTCGCCGTCCAGCTCGACCAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry