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pCNPpo6-H98A
(Plasmid #46087)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46087 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMVbeta
  • Backbone manufacturer
    clontech
  • Total vector size (bp) 4255
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PpoI H98A
  • Alt name
    PpoI
  • Species
    physarum polycephalum
  • Insert Size (bp)
    515
  • Mutation
    catalytically inactive H98A variant
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

See Addgene plasmid 46086 for description of wild type plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCNPpo6-H98A was a gift from Raymond Monnat (Addgene plasmid # 46087 ; http://n2t.net/addgene:46087 ; RRID:Addgene_46087)
  • For your References section:

    Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI. Monnat RJ Jr, Hackmann AF, Cantrell MA. Biochem Biophys Res Commun. 1999 Feb 5;255(1):88-93. 10.1006/bbrc.1999.0152 PubMed 10082660