|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46087||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 4255
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert namePpoI H98A
Insert Size (bp)515
Mutationcatalytically inactive H98A variant
- Promoter CMV
/ Fusion Protein
- NLS (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (destroyed during cloning)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
See Addgene plasmid 46086 for description of wild type plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCNPpo6-H98A was a gift from Raymond Monnat (Addgene plasmid # 46087 ; http://n2t.net/addgene:46087 ; RRID:Addgene_46087)
For your References section:Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI. Monnat RJ Jr, Hackmann AF, Cantrell MA. Biochem Biophys Res Commun. 1999 Feb 5;255(1):88-93. 10.1006/bbrc.1999.0152 PubMed 10082660