pG4-CMV-luc
(Plasmid #46322)

• Depositing Lab: Steven Brown
• Publication: Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. Epub 2012 Sep 10.

Backbone

• Vector backbone: pCMV-luc
• Total vector size (bp): 5530
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5xGal4
• Alt name: Gal4
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 122
• Mutation: five copies of a multimerized GAL4 (5×GAL4) site (cut from pFR-luc; Invitrogen)
• Promoter: CMV
• Tags / Fusion Proteins:
  • CMV promoter (C terminal on backbone)
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry