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Depositing Lab
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Depositing OrganizationIstituto di Biofisica, Consiglio Nazionale delle Ricerche
Located in Italy -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 46366-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 46366-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5363
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameClopHensor-H148G-V224L
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Alt nameE2GFP-H148G-V224L fused to DsRed-monomer
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Insert Size (bp)1485
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MutationE2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L
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Tag
/ Fusion Protein
- Strep-II (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 46366-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 46366-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-ClopHensor-H148G-V224L was a gift from Daniele Arosio (Addgene plasmid # 46366 ; http://n2t.net/addgene:46366 ; RRID:Addgene_46366) -
For your References section:
Calibration and functional analysis of three genetically encoded Cl(-)/pH sensors. Mukhtarov M, Liguori L, Waseem T, Rocca F, Buldakova S, Arosio D, Bregestovski P. Front Mol Neurosci. 2013 Apr 18;6:9. doi: 10.3389/fnmol.2013.00009. Print 2013. 10.3389/fnmol.2013.00009 PubMed 23616745