Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #46567)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 46567 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 7528
  • Total vector size (bp) 6184
  • Modifications to backbone
    1. EF1alpha promoter removed (so attR Gateway sites can be used to clone in synthetic promoters) 2. IVS-EGFP cassette inserted between attR Gateway cassette and BGH polyA 3. Synthetic promoters flanked by attL in pDONR221 (Life Technologies) vectors were used to recombine promoters into the vector resulting from modifications 1 and 2 above.
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Hygromycin ; No ATG until recombines into FRT site in target cell genome

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    CMV mutant promoter
  • Species
    Cytomegalovirus viral promoter
  • Insert Size (bp)
  • Mutation
    Mutant version of CMV promoter (refer to Table 1 for sequence and strength information)
  • GenBank ID

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCOCK CM-R03 was a gift from Clifford Wang (Addgene plasmid # 46567 ; ; RRID:Addgene_46567)
  • For your References section:

    Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Ferreira JP, Peacock RW, Lawhorn IE, Wang CL. Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20. 10.1007/s11693-011-9089-0 PubMed 23205156