pCOCK CM-R05
(Plasmid #46568)

• Purpose: Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).
• Depositing Lab: Clifford Wang
• Publication: Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.

Backbone

• Vector backbone: pEF5-FRT-V5-DEST
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 7528
• Total vector size (bp): 6182
• Modifications to backbone: 1. EF1alpha promoter removed (so attR Gateway sites can be used to clone in synthetic promoters) 2. IVS-EGFP cassette inserted between attR Gateway cassette and BGH polyA 3. Synthetic promoters flanked by attL in pDONR221 (Life Technologies) vectors were used to recombine promoters into the vector resulting from modifications 1 and 2 above.
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Hygromycin ; No ATG until recombines into FRT site in target cell genome

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV mutant promoter
• Species: Cytomegalovirus viral promoter
• Insert Size (bp): 587
• Mutation: Mutant version of CMV promoter (refer to Table 1 for sequence and strength information)
• GenBank ID:

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry