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pCOCK CM-R05
(Plasmid #46568)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46568 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF5-FRT-V5-DEST
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 7528
  • Total vector size (bp) 6182
  • Modifications to backbone
    1. EF1alpha promoter removed (so attR Gateway sites can be used to clone in synthetic promoters) 2. IVS-EGFP cassette inserted between attR Gateway cassette and BGH polyA 3. Synthetic promoters flanked by attL in pDONR221 (Life Technologies) vectors were used to recombine promoters into the vector resulting from modifications 1 and 2 above.
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Hygromycin ; No ATG until recombines into FRT site in target cell genome

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV mutant promoter
  • Species
    Cytomegalovirus viral promoter
  • Insert Size (bp)
    587
  • Mutation
    Mutant version of CMV promoter (refer to Table 1 for sequence and strength information)
  • GenBank ID

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCOCK CM-R05 was a gift from Clifford Wang (Addgene plasmid # 46568 ; http://n2t.net/addgene:46568 ; RRID:Addgene_46568)
  • For your References section:

    Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Ferreira JP, Peacock RW, Lawhorn IE, Wang CL. Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20. 10.1007/s11693-011-9089-0 PubMed 23205156