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pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1
(Plasmid #46676)


Item Catalog # Description Quantity Price (USD)
Plasmid 46676 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pcDNA3.2 V5-DEST mir-1-1 reporter
  • Backbone manufacturer
    Bartel lab (Addgene plasmid# 46646)
  • Backbone size w/o insert (bp) 8038
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    mir-133a-1 pri-mRNA
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIR133A1 (a.k.a. MIRN133A1, mir-133a-1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • hsa-mir-1-1 (C terminal on insert)
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1 was a gift from David Bartel (Addgene plasmid # 46676 ; ; RRID:Addgene_46676)
  • For your References section:

    Beyond Secondary Structure: Primary-Sequence Determinants License Pri-miRNA Hairpins for Processing. Auyeung VC, Ulitsky I, McGeary SE, Bartel DP. Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031. 10.1016/j.cell.2013.01.031 PubMed 23415231