Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #47916)


Item Catalog # Description Quantity Price (USD)
Plasmid 47916 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 6536
  • Modifications to backbone
    Puromycin resistance gene replaced with Nourseothricin resistance gene
  • Vector type
    Mammalian Expression, Retroviral
  • Promoter Tet
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original NAT ORF was obtained from Prof Laura Knoll, University of Wisconsin-Madison. Sequence of the ORF was partially adapted to human codon usage.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRXTN was a gift from James Iglehart (Addgene plasmid # 47916 ; ; RRID:Addgene_47916)
  • For your References section:

    Nourseothricin N-acetyl transferase: a positive selection marker for Mammalian cells. Kochupurakkal BS, Iglehart JD. PLoS One. 2013 Jul 4;8(7):e68509. doi: 10.1371/journal.pone.0068509. Print 2013. 10.1371/journal.pone.0068509 PubMed 23861913